Background. Diffuse gliomas are the most frequent and most dismal primary brain tumours in adults. Gliomas undergo cellular and molecular evolution during disease progression, which is only partially explained by genetic evolution, suggesting that transcriptional and epigenetic changes may be the basis of cellular adaptation processes. The international GLASS (Glioma Longitudinal AnalySiS) initiative, aims to systematically catalogue the temporal changes in gliomas at the genetic, epigenetic and transcriptomic levels. In this context, NORLUX aims to assess differential functional drug responses of primary versus recurrent gliomas.
Objectives. By combining mulitomics molecular characterisation and high content drug screens on ex vivo patient-derived glioma organoids from paired longitudinal tumors, we aim to determine differences in drug sensitivity and predict personalized treatment options for recurrent glioma patients. Patient-derived orthotopic xenografts (PDOX) will be applied for in vivo validation studies.
Training and research environment. The NORLUX lab is a dynamic, multinational and interdisciplinary team focusing on the biology of malignant brain cancer. It is part of the Department of Oncology and uses cutting edge technologies, including genomic, metabolomic and proteomic analyses, single cell technologies (single cell RNA-Seq, CYTOF, Imagestream) as well as state-of-the-art preclinical models. The project will be performed in collaboration with the international GLASS consortium (www.glass-consortium.org/). The post-doctoral fellow will be supported through a FNR CORE funded project and will join an interdisciplinary project team, including cellular and molecular biologists, animal experts, bioinformaticians and statisticians.