Novel regulators of GBM invasion identified by whole genome RNA interference screen

Invasion and angiogenesis are key characteristics of Glioblastoma and important targets for treatment. GBM cells display vastly different invasion capacities, which can be modeled in vivo and ex vivo. We have characterized the invasion behavior of GBM tumor cells in multiple patient-derived orthotopic xenografts (PODXs) in vivo, patient-derived GBM cell lines in the mouse brain (in vivo), in brain slice cultures (ex vivo) and in Boyden chamber assays (in vitro). Recently, we performed a RNA interference screen and have identified novel candidate genes that play a key role in GBM invasion. Functional validation of the top candidates essential for invasion within the brain are currently performed. Elucidating the molecular mechanisms of GBM invasion may provide opportunities to interfere with tumor cell spread and recurrence.

This project is supported by the Fondation Cancer Luxembourg and the Luxembourg National Research Fund (PRIDE15/10675146/CANBIO).

Key publications

Schuster et al., Nature Comms, 2020 pubmed
Fabian et al., Int Rev Cell Mol Biology, 2020 pubmed
Schuster et al., Trends Biotechnol, 2019 pubmed
Fack et al., Acta N, 2015 pubmed
Demeure et al., MCP, 2015 pubmed
Keunen et al., PNAS, 2011 pubmed
Rajcevic et al., MCP, 2009 pubmed